Gly-Pro-AMC

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Gly-Pro-AMC 功能研究：
Gly-Pro-AMC is a sensitive fluorogenic substrate for detecting dipeptidyl peptidase IV (DPPIV), a serine protease that cleaves N-terminal dipeptides from polypeptides with L-proline or L-alanine at the penultimate position. DPPIV is a multifunctional protein expressed on the surface of several cell types including epithelial, endothelial and lymphoid cells. It is identical to the T cell activation antigen CD26 and the adenosine deaminase binding protein, and it is also released as a soluble form in plasma. The substrates of CD26/DPPIV include a wide variety of proline-containing peptides such as growth factors, chemokines, neuropeptides, and vasoactive peptides. DPPIV is involved in immune regulation, signal transduction, and apoptosis, and appears to play an important role in tumor progression. Importantly, DPPIV is a therapeutic target for type II diabetes due to its role as a serum protease that cleaves incretin hormones of the glucagon family of peptides and thus regulates glucose homeostasis. Studies indicate that a DPPIV inhibitor improves impaired glucose tolerance. The assay of DPPIV with Gly-Pro-AMC is a simple, fast and flexible assay that is ideal for high-through put screening. The assay demonstrates improved sensitivity compared to colorimetric DPPIV assays, allowing the researcher to use less enzyme while still achieving appropriate Z' values. The homogeneous coupled-enzyme format is convenient, requiring only a single reagent addition to the test samples. Maximal sensitivity is achieved in 20-30 minutes, and the signal generated is very stable.

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Gly-Pro-AMC存儲(chǔ)液制備：
Gly-Pro-AMC 工作溶液：
※ 取出一個(gè)小瓶，用您選擇的分析緩沖液（例如 50 mM Tris-HCl、pH 7.5、1.0 mM DTT）稀釋儲(chǔ)備溶液，制成 50 到 100 µM 的分析溶液。
※ 將特定質(zhì)量Gly-Pro-AMC加入 DMSO 制備所需濃度體積的存儲(chǔ)液。 請(qǐng)注意，僅用于制備儲(chǔ)備溶液。 實(shí)驗(yàn)/緩沖液，請(qǐng)參閱示例實(shí)驗(yàn)協(xié)議。

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	225.545  µL	1.128 mL	2.255 mL	11.277 mL	22.555 mL
5 mM	45.109  µL	225.545  µL	451.091  µL	2.255 mL	4.511 mL
10 mM	22.555  µL	112.773  µL	225.545  µL	1.128 mL	2.255 mL

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Gly-Pro-AMC光譜圖：

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Gly-Pro-AMC 實(shí)驗(yàn)注意事項(xiàng)：
1.實(shí)驗(yàn)前需戴好防護(hù)眼鏡，穿戴防護(hù)服和口罩，佩戴手套，避免與皮膚接觸。
2.實(shí)驗(yàn)過程中如遇到有毒或者刺激性物質(zhì)及有害物質(zhì)產(chǎn)生，必要時(shí)實(shí)驗(yàn)操作需要手套箱內(nèi)完成以免對(duì)實(shí)驗(yàn)人員造成傷害
3.實(shí)驗(yàn)后產(chǎn)生的廢棄物需分類存儲(chǔ)，并交于專業(yè)生物廢氣物處理公司處理，以免造成環(huán)境污染Experimental considerations:
1. Wear protective glasses, protective clothing and masks, gloves, and avoid contact with the skin during the experiment.
2. The waste generated after the experiment needs to be stored separately, and handed over to a professional biological waste gas treatment company to avoid environmental pollution.